How and How Much Can Hoechst 33258 Cause Unwinding in a DNA Duplex?
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چکیده
منابع مشابه
How and how much can Hoechst 33258 cause unwinding in a DNA duplex?
The effect of Hoechst 33258 binding on the geometry of a DNA duplex (plasmid pBR322) has been examined using topoisomerase II relaxation followed by gel electrophoresis. Of this drug-DNA system, fluorescence, optical absorption, and calorimetric measurements were also made at various drug and DNA concentrations and in the same buffer as that for the topoisomerase reaction. It has been confirmed...
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An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
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Buoyant density of DNA in CsCl gradients with Hoechst 33258 (bisbenzimide) was investigated as a function of guanine plus cytosine content of the DNA (%GC; in mole percent). A formula for calculating %GC from the refractive index (nD) of the isopycnic CsCl/Hoechst 33258 solution over the range of 0-75 %GC was established: %GC = 351762.28 X nD - 123778.66 X nD2 - 249789.47 (the coefficients must...
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We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1999
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.47.1363